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crude protein solution  (TaKaRa)


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    TaKaRa crude protein solution
    Crude Protein Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crude+protein+solution/Capturem+Protein+A+Buffer/bio_rxiv__2025__01__11__632565-265-2-14
    Average 92 stars, based on 5 article reviews
    crude protein solution - by Bioz Stars, 2026-09
    92/100 stars

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    Purification:

    Article Title: Epigenetic phase variation in the gut microbiome enhances bacterial adaptation
    Article Snippet: The supernatant from cell culture was concentrated to 1 ml using an Amicon Ultra Centrifugal Filter unit (3 kDa, Millipore) and subjected to sonication as described above. .. The resulting crude protein solution was purified using a Capturem His-Tagged Purification kit (#635719, TaKaRa Bio Inc.) following the manufacturer’s instructions. .. Protein samples were mixed with 4× Sodium dodecyl sulfate (SDS) loading buffer (Bio-Rad Laboratories, Inc) and heated at 98°C for 5 min. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using Mini-PROTEIN TGX Gels (4–20%, Bio-Rad Laboratories, Inc).



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    Schematic of the autoantibody screening pipeline. In the first step, proteins were synthesized in vitro from the proteome-wide human cDNA library (HuPEX). Promotors (P), Enhancers (E), and FLAG-GST tags were fused to open reading frames of the expression clones by Gateway LR reaction. After polymerase chain reaction amplification and in vitro transcription, translation was performed using the wheat germ cell-free synthesis system. In the second step, we conducted autoantibody screening by treating proteome-wide wet protein arrays with pooled serum. In the third step, autoantibody quantification was performed by treating focused wet protein arrays with individual sera. ORF, open reading frame; SSc, systemic sclerosis; Pso, psoriasis; CA, cutaneous arteritis; MM, malignant melanoma; Ctrl, healthy control.

    Journal: Frontiers in Immunology

    Article Title: Autoantibody Landscape Revealed by Wet Protein Array: Sum of Autoantibody Levels Reflects Disease Status

    doi: 10.3389/fimmu.2022.893086

    Figure Lengend Snippet: Schematic of the autoantibody screening pipeline. In the first step, proteins were synthesized in vitro from the proteome-wide human cDNA library (HuPEX). Promotors (P), Enhancers (E), and FLAG-GST tags were fused to open reading frames of the expression clones by Gateway LR reaction. After polymerase chain reaction amplification and in vitro transcription, translation was performed using the wheat germ cell-free synthesis system. In the second step, we conducted autoantibody screening by treating proteome-wide wet protein arrays with pooled serum. In the third step, autoantibody quantification was performed by treating focused wet protein arrays with individual sera. ORF, open reading frame; SSc, systemic sclerosis; Pso, psoriasis; CA, cutaneous arteritis; MM, malignant melanoma; Ctrl, healthy control.

    Article Snippet: Wheat germ cell-free crude solution expressing the target protein harboring FLAG-GST-tag was diluted and spotted onto GSH-coated glass-plates using a HORNET-NX multi-dispense system (Wako Pure Chemical Industries).

    Techniques: Synthesized, In Vitro, cDNA Library Assay, Expressing, Clone Assay, Polymerase Chain Reaction, Amplification